post Homebrew digital microscope

Filed under Electronics by Iain (9:15 pm, November 18th, 2009)

This is a fun little project I finished a few evenings ago; a homebrew 500x digital microscope for all your biohacking needs, made from an old webcam found in the trusty junk box.

microscope

Read on for a mini-howto and some captured microscopic images.

A webcam usually uses just a single lens focusing straight onto the CCD imager. And usually, the lens is mounted in a little rotating bezel. By removing the lens bezel and reversing it, so that the lens is much futher from the CCD than usual, the focal length is reduced to a few millimeters in front of the lens – making for a surprisingly usable microscope.

You can see the arrangement in the pictures; I just taped the lens assembly on backwards having unscrewed it from the mount.

I removed the green LED the webcam had and used the handy 5V to power three white LEDS mounted below the slide platform (I think that’s called the stage on real microscopes). The LEDs have a little switch to turn them on and off, as sometimes you want your specimen lit from above instead of below. They’re mounted on a blob of bluetack to allow the beams to be adjusted easily.

The slide platform itself is a piece of transparent plastic epoxied onto the top of a large bolt, which is screwed into a fixed nut. This allows me to bring the slide into perfect focus by rotating the platform and bolt. Real microscopes focus by moving the lens rather than the specimen, but this was easier to constuct and although a little fiddly in use becomes easy with practice.

microscope_in_use

The quality of the images obtained was a lot higher than I expected – check them out below. Magnification is around 500x and it’s possible to see animal cells. Click an image to enlarge it.

Eel cells   Eel cells

Fly wing   Human hair

Highly recommended as something to make on a rainy weekend :)

2 Comments »

  1. That is a slick unit! I’m impressed you were able to get that out of a webcam! I think you may also find benefit by putting a frosted glass object (like a slide between the LEDs and the stage. You’d have to play with it to see if it actually helps, but my experience with microscopes is that it is a very rare sample that prefers undiffused light.

    Zack

    Comment by Zack Gainsforth — Jan 26 2010 @ 5:43 am

  2. hey !
    I really like your digital microscope- it looks really interesting – just what i need for msd work :)
    I wish to know what megapixel camera you have used, and also possibly what make.
    Congrats on your cool website- keep it upi !
    greetings from ireland,
    hamman

    Comment by hamman — Mar 4 2010 @ 8:42 pm

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